GCMS, phytochemicals and antioxidant activities of in vitro callus extracts of Strobilanthes kunthiana (Nees) T. Anderson ex Benth: An endemic plant of Acanthaceae

Resumo

Strobilanthes kunthiana (Neelakurinji) is an endemic
and underexploited plant belongs to the Family Acanthaceae.
The aim of our study was to evaluate phytochemical analysis,
GC-MS analysis and antioxidant activity of in vitro callus extract
of Strobilanthes kunthiana. In this present study the
phytochemical analysis of various extract of S. kunthiana in vitro
callus were studied. Phytochemical analysis confirmed the
presence of alkaloids, flavonoids, glycosides, saponins, tannins,
steroids, terpenoids and phenols in the methanolic extract
comparing to the other extracts. In this study GC-MS analysis
revealed the presence of 10 bioactive phytochemical compounds
were identified in the methanolic extract. The prevailing
compounds were 9,12-octadecadienoic acid (Z,Z) (50.32%),
hexadecanoic acid, methyl ester (20.69%), 9-octadecenoic acid
(Z)-,methyl ester (10.45%), heptadecanoic acid, 16-methyl-,
methyl ester (5.78%), 2,6-bis(1,1-dimethylethyl)-4-methyl
phenol (4.70%). 3-methyl-2-ketobutyric acid tbdms (2.79%),
2,2,3,4-tetramethyl-5-hexen-3-ol
(2.41%),
butoxycarbonyl)-2-(4-methoxyphenyl)
allylamine
N-(tert
(1.06%),
cyclotrisiloxane, hexamethyl (0.94%), benzenesulfonamide
(0.87%). The antioxidant property was evaluated for methanol
and ethanolic extract by DPPH method. The higher percentage of
inhibition (79.23 ± 0.37) was observed in 250 µg/mL of ethanol
extract followed by (90.35 ± 0.54) methanolic extract against the
standard ascorbic acid (91.25 ± 0.33). The results show that the
methanolic extract possesses more antioxidant activity than
ethanol. The plant S. kunthiana may be exploited as a source of
natural antioxidant and as herbal alternatives for various
disorders.

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