The potential of naturally occurring bacteria for the bioremediation of toxic metals pollution

Resumo

An increase in industrialization and various kind of human activities added a huge amount of toxic heavy metals in the soil. As a result, toxic heavy metals in the environment may be adversely affects human being and aquatic ecosystem. Thus, it is very essential to understand mechanism of bioremediation through eco-friendly agent i.e. bacteria. Accumulation of high metal concentrations in soil above threshold limit causes lethal to bacterial communities in the environment. Few bacteria develop resistance mechanism to tolerate these toxic heavy metals and contain various methods to respond the metal stress. The present review emphasizes to understand the mechanism of bacterial resistance against toxic metals. Moreover, mechanism of bioaugmentation, biosorption, and bioaccumulation methods also described clearly.

Referências

1. Agarry, S. E.; Owabor, C. N. Anaerobic
2. bioremediation of marine sediment
3. artificially contaminated with anthracene and
4. naphthalene. Environmental Technology,
5. v. 32, p. 1375-1381, 2011.
6. Agarwal, S. K. Environmental
7. Biotechnology. 1. ed. New Delhi, India: APH
8. Publishing Corporation, 1998.
9. Ahemad, M.; Khan, M. S.; Zaidi, A.; Wani, P. A.
10. Remediation of herbicides contaminated soil
11. using microbes. In: Khan, M. S.; Zaidi, A.;
12. Musarrat, J. (Eds.). Microbes in sustainable
13. agriculture. New York: Nova Science
14. Publishers, 2009. p. 261-284.
15. Akar, T.; Tunali, S.; Cabuk, A. Study on the
16. characterization of lead(II) biosorption by
17. The potential of naturally occurring bacteria for the bioremediation 47
18. Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 39-51.
19. fungus Aspergillus parasiticus. Applied
20. Biochemistry and Biotechnology, v. 136,
21. p. 389-406, 2007.
22. Anon. Bioremediation of Alaskan sites on the
23. way. Oil & Gas Journal, v. 4, p. 42-46, 1990.
24. Atlas, R. M. Petroleum biodegradation and oil
25. spill bioremediation. Marine Pollution
26. Bulletin, v. 31, p. 178-182, 1995.
27. ATSDR - Agency for Toxic Substances and
28. Disease Registry. Toxicological profile for
29. lead. Atlanta: US Department of Health and
30. Human Services, 2007.
31. Bae, W.; Mehra, R. K.; Mulchandani, A.; Chen,
32. W. Genetic engineering of Escherichia coli for
33. enhanced uptake and bioaccumulation of
34. mercury. Applied and Environmental
35. Microbiology, v. 67, p. 5335-5338, 2001.
36. Bae, W.; Wu, C. H.; Kostal, J.; Mulchandani, A.;
37. Chen, W. Enhanced mercury biosorption by
38. bacterial cells with surface-displayed MerR.
39. Applied and Environmental Microbiology,
40. v. 69, p. 3176-3180, 2003.
41. Al-Baldawi, I. A.; Abdullah, S. R. S.; Anuar.N.;
42. Suja, F.; Mushrifah, I. Phytodegradation of
43. total petroleum hydrocarbon (TPH) in dieselcontaminated water using Scirpus grossus.
44. Ecological Engineering, v. 74, p. 463-473,
45. Barnhart, M. J.; Meyers, J. M. Pilot
46. bioremediation tells all about petroleum
47. contaminated soil. Pollution Engineerings,
48. v. 21, p. 110-112, 1989.
49. Bartha, R.; Bossert, I. The treatment and
50. disposal of petroleum refinery wastes. In:
51. Atlas, R.M. (Ed.). Petroleum microbiology.
52. New York, USA: Macmillan Publishing, 1984.
53. p. 553-578.
54. Bellinger, D. C.; Bellinger, A. M. Childhood
55. lead poisoning: The torturous path from
56. science to policy. Journal of Clinical
57. Investigation, v. 116, p. 853-857, 2006.
58. Beolchini, F.; Rocchetti, L.; Regoli, F.;
59. Dell’Anno, A. Bioremediation of marine
60. sediments contaminated by hydrocarbons:
61. Experimental analysis and kinetic modeling.
62. Journal of Hazardous Materials, v. 182,
63. p. 403-407, 2010.
64. Brierley, C. L. Bioremediation of metalcontaminated surface and groundwater.
65. Geomicrobiology Journal, v. 8, p. 201-223,
66. Brown, G. E. J.; Foster, A. L.; Ostergren, J. D.
67. Mineral surfaces and bioavailability of heavy
68. metals: A molecular-scale perspective. PNAS,
69. v. 96, p. 3388-3395, 1999.
70. Bruschi, M.; Goulhen, F. New bioremediation
71. technologies to remove heavy metals and
72. radionuclides using Fe (III)-sulfate- and
73. sulfur reducing bacteria. In: Singh, S. N.;
74. Tripathi, R. D. (Eds.). Environmental
75. Bioremediation Technologies. NY, USA:
76. Springer, 2006. p. 35-55.
77. Carter, P.; Cole, H, Burton, J. Bioremediation:
78. Successes and shortfalls. Proceedings of Key
79. Bioremediation Process, 2006.
80. Chaudri, A. M.; McGrath, S. P.; Giller, K. E.;
81. Rietz, E.; Sauerbeck, D. R. Enumeration of
82. indigenous Rhizobium leguminosarum biovar
83. trifolii in soils previously treated with metalcontaminated sewage sludge. Soil Biology
84. and Biochemistry, v. 25, p. 301-309, 1993.
85. Chen, C.; Wang, J. L. Characteristics of Zn2+
86. biosorption by Saccharomyces cerevisiae.
87. Biomedical and Environmental Sciences,
88. v. 20, p. 478-482, 2007.
89. Cobbett, C.; Goldsbrough, P. Phytochelatins
90. and metallothioneins: roles in heavy metal
91. detoxification and homeostasis. Annual
92. Review of Plant Biology, v. 53, p. 159-182,
93. Comte, S.; Guibaud, G.; Baudu, M. Biosorption
94. properties of extracellular polymeric
95. substances (EPS) towards Cd, Cu and Pb for
96. different pH values. Journal of Hazardous
97. Materials, v. 151, p. 185-193, 2008.
98. D’Annibale, A.; Leonardi, V.; Federici, E.;
99. Baldi, F.; Zecchini, F.; Petruccioli, M. Leaching
100. and microbial treatment of a soil
101. contaminated by sulphide ore ashes and
102. aromatic hydrocarbons. Applied
103. Microbiology and Biotechnology, v. 74,
104. p. 1135-1144, 2007.
105. Das, N.; Vimala, R.; Karthika, P. Biosorption of
106. heavy metals: An overview. Indian Journal
107. of Biotechnology, v. 7, p. 159-169, 2008.
108. Dell’Anno, A.; Beolchini, F.; Rocchetti, L.;
109. Luna, G. M.; Danovaro, R. High bacterial
110. biodiversity increases degradation
111. performance of hydrocarbons during
112. bioremediation of contaminated harbour
113. marine sediments. Environmental
114. Pollution, v. 167, p. 85-92, 2012.
115. Vashishth et al.
116. Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 39-51.
117. Divya, B.; Deepak, K. M. Plant-microbe
118. interaction with enhanced bioremediation.
119. Research Journal of BioTechnology, v. 6,
120. p. 72-79, 2011.
121. EFSA - European Food Safety Authority.
122. Cadmium in food: Scientific opinion of the
123. panel on contaminants in the food chain.
124. EFSA Journal, v. 7, no. 3, 2009.
125. https://doi.org/10.2903/j.efsa.2009.980
126. Evanko, C. R.; Dzombak, D. A. Remediation of
127. metals-contaminated soil and groundwater.
128. Environmental Sciences, v. 412, p. 1-45,
129. Fan, M. Y.; Xie, R. J.; Qin, G. Bioremediation of
130. petroleum-contaminated soil by a combined
131. system of biostimulation-bioaugmentation
132. with yeast. Environmental Technology,
133. v. 35, no. 1/4, p. 391-399, 2013.
134. Fang, L. C.; Huang, Q. Y.; Wei, X.; Liang, W.;
135. Rong, X. M.; Chen, W. L.; Cai, P.
136. Microcalorimetric and potentiometric
137. titration studies on the adsorption of copper
138. by extracellular polymeric substances (EPS),
139. minerals and their composites. Bioresource
140. Technology, v. 101, p. 5774-5779, 2010.
141. Fang, L.; Wei, X.; Cai, P.; Huang, Q.; Chen, H.;
142. Liang, W.; Rong, X. Role of extracellular
143. polymeric substances in Cu(II) adsorption on
144. Bacillus subtilis and Pseudomonas putida.
145. Bioresource Technology, v. 102, p. 1137-
146. , 2011.
147. Gadd, G. M. Metals and microorganisms: A
148. problem of definition. FEMS Microbiology
149. Letters, v. 100, p. 197-204, 1992.
150. Gan, S.; Lau, E. V.; Ng, H. K. Remediation of
151. soils contaminated with polycyclic aromatic
152. hydrocarbon biodegradation. Journal of
153. Hazardous Materials, v. 172, p. 532-549,
154. Garbisu, C.; Alkorta, I. Phytoextraction: A
155. cost-effective plant-based technology for the
156. removal of metals from the environment.
157. Bioresource Technology, v. 77, p. 229-236,
158. Gaspare, L.; John, F.; Machiwa, S. J. M.; Streck,
159. G.; Brack, W. Polycyclic aromatic
160. hydrocarbon (PAH) contamination of surface
161. sediments and oysters from the inter-tidal
162. areas of Dar es Salaam, Tanzania.
163. Environmental Pollution, v. 157, p. 24-34,
164. Giller, K. E.; Witter, E.; McGrath, S. P. Toxicity
165. of heavy metals to microorganisms and
166. microbial process in agricultural soils: A
167. review. Soil Biology and Biochemistry,
168. v. 30, p. 1389-1414, 1998.
169. Gómez Jiménez-T, R.; Moliterni, R.;
170. Rodríguez, E.; Fernández, L.; Villaseñor, F. J.
171. Feasibility of mixed enzymatic complexes to
172. enhanced soil bioremediation processes.
173. Procedia Environmental Sciences, v. 9,
174. p. 54-59, 2011. https://doi.org/10.1016/
175. j.proenv.2011.11.010
176. Gray, E. J.; Smith, D. L. Intracellular and
177. extracellular PGPR: Commonalities and
178. distinctions in the plant-bacterium signaling
179. processes. Soil Biology and Biochemistry,
180. v. 37, p. 395-412, 2005.
181. Guine, V.; Spadini, L.; Sarret, G.; Muris, M.;
182. Delolme, C.; Gaudet, J. P.; Martins, J. M. Zinc
183. sorption to three gram-negative bacteria:
184. Combined titration, modeling and EXAFS
185. study. Environmental Science &
186. Technology, v. 40, p. 1806-1813, 2006.
187. Gurer, H.; Ercal, N. Can antioxidants be
188. beneficial in the treatment of lead poisoning?
189. Free Radical Biology & Medicine, v. 29,
190. p. 927-945, 2000.
191. Haritash, A. K.; Kaushik, C. P. Biodegradation
192. aspects of polycyclic aromatic hydrocarbons
193. (PAHs): A review. Journal of Hazardous
194. Materials, v. 169, no. 1/3, p. 1-15, 2009.
195. https://doi.org/10.1016/j.jhazmat.2009.03.1
196. Hassan, I.; Mohamedelhassan, E.; Ernest, K.;
197. Yuan, Z. C. A Review article: Electrokinetic
198. bioremediation current knowledge and new
199. prospects. Advances in Microbiology, v. 6,
200. p. 57-72, 2016. https://doi.org/10.4236/
201. aim.2016.61006
202. Head, I. M.; Jones, D. M.; Roling, W. F. M.
203. Marine microorganisms make a meal of oil.
204. Nature Reviews Microbiology, v. 4, p. 173-
205. , 2006.
206. Head, I. M.; Swannell, R. P. J. Bioremediation
207. of petroleum hydrocarbon. Current Opinion
208. in Biotechnology, v. 3, p. 234-239, 1999.
209. Hernberg, S.; Nikkanen, J. Enzyme inhibition
210. by lead under normal urban conditions.
211. Lancet, v. 10, p. 63-64, 1970.
212. Hess, A.; Zarda, B.; Hahn, D.; Hanner, A.; Stax,
213. D. In situ analysis of denitrifying toluene and
214. mxylene degrading bacteria in a diesel fuel
215. The potential of naturally occurring bacteria for the bioremediation 49
216. Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 39-51.
217. contaminated laboratory aquifer column.
218. Applied and Environmental Microbiology,
219. v. 63, p. 2136-2141, 1997.
220. Huckle, J. W.; Morby, A. P.; Turner, J. S.;
221. Robinson, N. J. Isolation of a prokaryotic
222. metallothionein locus and analysis of
223. transcriptional control by trace metal ions.
224. Molecular Microbiology, v. 7, p. 177-187,
225. Igiri, B. E.; Okoduwa, S. I. R.; Idoko, G. O.;
226. Akabuogu, E. P.; Adeyi, A. O.; Ejiogu, I. K.
227. Toxicity and Bioremediation of heavy metals
228. contaminated ecosystem from tannery
229. wastewater. Journal of Toxicology, v. 2018,
230. Article ID 2568038, 2018.
231. https://doi.org/10.1155/2018/2568038
232. Ijah, U. J. J. Accelerated crude oil
233. biodegradation in soil by inoculation with
234. bacterial slurry. Journal of Environmental
235. Sciences, v. 1, p. 38-47, 2002.
236. Ijah, U. J. J. The potential use of chickendrop
237. microorganisms for oil spill remediation. The
238. Environmentalist, v. 23, p. 89-95, 2003.
239. Ijah, U. J. J.; Antai, S. P. Degradation and
240. mineralization of crude oil by bacteria.
241. Nigerian Journal of Biotechnology, v. 5,
242. p. 79-87, 1988.
243. Janjua, N. Z.; Kasi, P. M.; Nawaz, H.; Farooqui,
244. S. Z.; Khuwaja, U. B.; Hassan, N.; Jafri, S. N.;
245. Lutfi, S. A.; Kadir, M. M.; Sathiakumar, N.
246. Acute health effects of the Tasman Spirit oil
247. spill on residents of Karachi, Pakistan. BMC
248. Public Health, 6:84, 2006.
249. https://doi.org/10.1186/1471-2458-6-84
250. Jarup, L. Hazards of heavy metal
251. contamination. British Medical Bulletin,
252. v. 68, p. 167-182, 2003.
253. Kamaludeen, S. P. B. K.; Arunkumar, K. R.;
254. Avudainayagam, S.; Ramasamy, K.
255. Bioremediation of chromium contaminated
256. environments. International Journal of
257. Experimental Biology, v. 41, p. 972-985,
258. Kao, P. H.; Huang, C. C.; Hseu, Z. Y. Response
259. of microbial activities to heavy metals in a
260. neutral loamy soil treated with biosolid.
261. Chemosphere, v. 64, p. 63-70, 2006.
262. Kelly, D. J. A.; Budd, K.; Lefebvre, D. D. The
263. biotransformation of mercury in pH-stat
264. cultures of microfungi. Canadian Journal of
265. Botany, v. 84, p. 254-260, 2006.
266. Khan, M. S.; Zaidi, A.; Wani, P. A.; Oves, M.
267. Role of plant growth promoting rhizobacteria
268. in the remediation of metal contaminated
269. soils. Environmental Chemistry Letters,
270. v. 7, p. 1-19, 2009.
271. Kinya, K.; Kimberly, L. D. Current use of
272. bioremediation for TCE cleanup: Results of a
273. survey. Remediation Journal, v. 6, p. 1-14,
274. Kumar, A.; Bisht, B. S.; Joshi, V. D.; Dhewa, T.
275. Review on bioremediation of polluted
276. environment: A management tool.
277. International Journal of Environmental
278. Sciences, v. 1, p. 1079-1093, 2011.
279. Law, R. J.; Klungsoyr, J. The analysis of
280. polycyclic aromatic hydrocarbons in marine
281. samples. International Journal of
282. Environmental Policy and Decision
283. Making, v. 13, p. 262-283, 2000.
284. Lloyd, J. R.; Lovley, D. R. Microbial
285. detoxification of metals and radionuclides.
286. Current Opinion in Biotechnology, v. 12,
287. p. 248-253, 2001.
288. Lovely, D. R. Dissimilatory metal reduction:
289. From early life to bioremediation. ASM
290. News, v. 68, p. 231-237, 2002.
291. Lovley, D. R.; Philips, E. J.; Gorby, Y. A.; Landa,
292. E. R. Microbial reduction of uranium. Nature,
293. v. 350, p. 413-416, 1991.
294. Lovley, D. R.; Phillips, E. J. P. Novel mode of
295. microbial energy metabolism: Organic
296. carbon oxidation to dissimilatory reduction
297. of iron or manganese. Applied and
298. Environmental Microbiology, v. 54, p.
299. -1480, 1988.
300. Lyons, R. A.; Temple, J. M. F.; Evans, D.; Fone,
301. D. L.; Palmer, S. R. Acute health effects of the
302. sea empress oil spill. Journal of
303. Epidemiology and Community Health, v.
304. , p. 306-310, 1999.
305. Mejare, M.; Bulow, L. Metal-binding proteins
306. and peptides in bioremediation and
307. phytoremediation of heavy metals. Trends in
308. Biotechnology, v. 19, p. 67-73, 2001.
309. Millar, J. A.; Battistini, V.; Cumming, R. L. C.;
310. Carswell, F.; Goldberg, A. Lead and daminolevulinic acid dehydratase levels in
311. mentally retarded children and in leadpoisoned suckling rats. Lancet, v. 3, p. 695-
312. , 1970.
313. Vashishth et al.
314. Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 39-51.
315. Miura, N. Individual susceptibility to
316. cadmium toxicity and metallothionein gene
317. polymorphisms with reference to current
318. status of occupational cadmium exposure.
319. Industrial Health, v. 47, p. 487-494, 2009.
320. Needleman, H. Lead poisoning. Annual
321. Review of Medicine, v. 55, p. 209-222, 2004.
322. Nies, D. H. Microbial heavy metal resistance.
323. Applied Microbiology and Biotechnology,
324. v. 51, p. 730-750, 1999.
325. O’Brien, P. Y.; Dixon, P. S. The effects of oils
326. and oil components on algae: A review.
327. British Phycological Journal, v. 11, p. 115-
328. , 1976.
329. Odu, C. T. I. Fermentation characteristics and
330. biochemical reactions of some organisms
331. isolated from oil polluted soils.
332. Environmental Pollution, v. 15, p. 271-276,
333. Okpokwasili, G. C.; Okorie, B. B. Biodeterioration potentials of microorganisms isolated
334. from engine lubricating oil. Tribology
335. International, v. 21, p. 215-220, 1988.
336. Oliveira, N. C.; Rodrigues, A. A.; Alves, M. I. R.;
337. Antoniosi Filho, N. R.; Sadoyama, G.; Vieira, J.
338. D. G. Endophytic bacteria with potential for
339. bioremediation of petroleum hydrocarbons
340. and derivatives. African Journal of
341. Biotechnology, v. 12, p. 2977-2984, 2012.
342. Olson, J. W.; Mehta, N. S.; Maier, R. J.
343. Requirement of nickel metabolism protein
344. HypA and HypB for full activity of both
345. hydrogenase and urease in Helicobacter
346. pylori. Molecular Microbiology, v. 39, p.
347. -182, 2001.
348. Onwubuya, K.; Cundy, A.; Puschenreiter, M.;
349. Kumpiene, J.; Bone, B. Developing decision
350. support tools for the selection of “gentle”
351. remediation approaches. Science of the
352. Total Environment, v. 407, p. 6132-6142,
353. Pinedo, R. C.; Aleu, J.; Collado, I. G. Pollutants
354. biodegradation by fungi. Current Organic
355. Chemistry, v. 13, p. 1194-1214, 2009.
356. Pritchard, P. H. Bioremediation as a
357. technology; experiences with the Exxon
358. Valdez spill. Journal of Hazardous
359. Materials, v. 28, p. 76-79, 1991.
360. Pritchard, P. H.; Costa, C. F. EPA’s Alaska oil
361. spill bioremediation project. Environmental
362. Science & Technology, v. 25, p. 115-130,
363. Roane, T. M.; Pepper, I. L. Microorganisms
364. and metal pollution. In: Maier, R. M.; Pepper,
365. I. L.; Gerba, C. B. (Eds.). Environmental
366. microbiology. London: Academic Press,
367. Saadoun, I. M. K.; Al-Ghzawi, Z. D. Bioremediation of petroleum contamination. In:
368. Fingerman, M.; Nagabhushanam, R. (Eds.).
369. Bioremediation of aquatic and terrestrial
370. ecosystems. Enfield, USA: Science
371. Publishers, 2005.
372. Saranya, K.; Sundaramanickam, A.; Shekhar,
373. S.; Swaminathan, S.; Balasubramanian, T.
374. Bioremediation of mercury by Vibrio fluvialis
375. screened from industrial effluents. BioMed
376. Research International, v. 12, p. 1-6, 2017.
377. Sayler, G. S.; Ripp, S. Field applications of
378. genetically engineered microorganisms for
379. bioremediation process. Current Opinion in
380. Biotechnology, v. 11, p. 286-289, 2000.
381. Sikkema, J.; Bont, J. A.; Poolman, B.
382. Mechanisms of membrane toxicity of
383. hydrocarbons. Microbiological Reviews,
384. v. 59, p. 201-222, 1995.
385. Silver, S. Bacterial heavy metal resistance:
386. New surprises. Annual Review of
387. Microbiology, v. 50, p. 753-789, 1996.
388. Singh, S.; Kang, S. H.; Mulchandani, A.; Chen,
389. W. Bioremediation: Environmental cleanup
390. through pathway engineering. Current
391. Opinion in Biotechnology, v. 19, p. 437-444,
392. Sloan, R. Bioremediation demonstrated at a
393. hazardous waste site. Oil & Gas Journal, v. 5,
394. p. 61-66, 1987.
395. Smith, V. H.; Graham, D. W.; Cleland, D. D.
396. Application of resource ratio theory to
397. hydrocarbon biodegradation. Environmental Science & Technology, v. 32,
398. p. 3386-3395, 1998.
399. Spormann, A. M.; Widdel, F. Metabolism of
400. alkyl benzenes, alkanes, and other
401. hydrocarbons in anaerobic bacteria.
402. Biodegradation, v. 11, p. 85-105, 2000.
403. Sposito, F. G. The chemistry of soils. In: Maier,
404. R. M.; Pepper, I. L.; Gerba, C. B. (Eds.).
405. Environmental microbiology. London:
406. Academic Press, 2000.
407. The potential of naturally occurring bacteria for the bioremediation 51
408. Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 39-51.
409. Strong, P. J.; Burgess, J. E. Treatment methods
410. for winerelated ad distillery wastewaters:
411. A review. Bioremediation Journal, v. 12,
412. p. 70-87, 2008.
413. Talos, K.; Pager, C.; Tonk, S.; Majdik, C.;
414. Kocsis, B.; Kilar, F.; Pernyeszi, T. Cadmium
415. biosorption on native Saccharomyces
416. cerevisiae cells in aqueous suspension. Acta
417. Universitatis Sapientiae, Agriculture and
418. Environment, v. 1, p. 20-30, 2009.
419. Tang, C. Y.; Criddle, Q. S.; Fu, C. S.; Leckie, J. O.
420. Effect of flux (transmembrane pressure) and
421. membranes properties on fouling and
422. rejection of reverse osmosis and
423. nanofiltration membranes treating
424. perfluorooctane sulfonate containing waste
425. water. Journal of Environmental Science
426. and Technology, v. 41, p. 2008-2014, 2007.
427. Thavasi, R. Microbial biosurfactants: From an
428. environment application point of view.
429. Journal of Bioremediation and
430. Biodegradation, v. 2, 100104e, 2011.
431. Tigini, V.; Prigione, V.; Giansanti, P.;
432. Mangiavillano, A.; Pannocchia, A.; Varese, G.
433. C. Fungal biosorption, an innovative
434. treatment for the decolourisation and
435. detoxification of textile effluents. Water, v. 2,
436. p. 550-565, 2010.
437. Tunali, S.; Akar, T.; Oezcan, A. S.; Kiran, I.;
438. Oezcan, A. Equilibrium and kinetics of
439. biosorption of lead(II) from aqueous
440. solutions by Cephalosporium aphidicola.
441. Separation and Purification Technology,
442. v. 47, p. 105-112, 2006.
443. Umrania, V. V. Bioremediation of toxic heavy
444. metals using acido-thermophilic autotrophes.
445. Bioresource Technology, v. 97, no. 10, p.
446. -1242, 2006. https://doi.org/10.1016/
447. j.biortech.2005.04.048
448. Valls, M.; Atrian, S. L. V.; La, F. Engineering a
449. mouse metallothionein on the cell surface of
450. Ralstonia eutropha CH34 for immobilization
451. of heavy metals in soil. Nature
452. Biotechnology, v. 18, p. 661-665, 2000.
453. Venosa, A. D.; Lee, K.; Suidan, M. T.; Garcia, B.
454. S.; Cobanli, S.; Moteleb, M.; Haines, J. R.;
455. Tremblay, G.; Hazelwood, M. Bioremediation
456. and biorestoration of a crude oil
457. contaminated freshwater wetland on the St.
458. Lawrence River. Biomedical Journal, v.6, p.
459. -281, 2002.
460. Verma, N.; Singh, M. Biosensors for heavy
461. metals. BioMetals, v. 18, no. 1, p. 121-129,
462. Wu, T.; Xie, W. J.; Yi, L.; Li, X. B.; Yang, B. H.;
463. Wang, J. Surface activity of salt-tolerant
464. Serratia spp. and crude oil biodegradation in
465. saline soil. Plant Soil Environ, v. 58, p. 412-
466. , 2012.