Production of Carboxymethyl Cellulase by Thermophilic Bacillus Licheniformis SMIA-2 Using Pretreated Sugarcane Bagasse and Some Properties of The Enzymatic Activity

Resumo

Agricultural wastes such as sugarcane bagasse are most frequently employed substrates for microbial cellulase production. It is abundantly and cheaply available as a byproduct from the sugar industry in Brazil. In this work, optimization techniques were studied using optimization techniques to optimize the components of the culture medium and some enzymatic parameters for the maximum activity of carboxymethyl cellulase (CMCase) by Bacillus licheniformis SMIA-2. For enzyme production, sugarcane bagasse pretreated with alkali and corn steep liquor was used as a carbon and nitrogen source, respectively. Some properties of the enzyme were also determined. The growth of Bacillus licheniformis SMIA-2 in submerged culture containing 0.625 % (w/v) SCB and 0.625 % (w/v) CSL for 120 h was considered the most effective for CMCase secretion. The optimal CMCase activity was obtained by incubating the enzyme at 70ºC and pH 8.0 for 10 minutes. The CMCase was stable over a wide pH range and at 60ºC for 1 h. In the presence of CoCl₂, the CMCase was stable at 70ºC for 1 h, and at 90ºC, the enzyme still retained more than 70% of its original activity. The great resistance to surfactants, oxidizing agents, and commercial detergents widens the potential application spectrum of CMCase from Bacillus sp. SMIA-2 in more fields, such as the detergent industry.

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