Abstract
This study evaluated the reproductive performance of female Travancoriana schirnerae Bott, 1969 (Crustacea: Gecarcinucidae) administered with 20-OH ecdysone during different phases of the oogenic cycle. The effect of administration was evaluated by comparing the mean gonadosomatic index, oocyte diameter, oocyte proportion values and histological features of the control and concurrent control ovaries with those of the experimentals. The results clearly indicated that 20-OH ecdysone can stimulate ovarian growth and maturation in all phases of the oogenic cycle, though it caused statistically significant effects only during the early and middle vitellogenic phases, evidenced from the accelerated gonadosomatic index, oocyte diameter and oocyte proportion values, occurrence of vitellogenic oocytes in avitellogenic and previtellogenic ovaries, increased degree of yolk deposition and proliferation of gonia in the experimental ovaries compared to the controls. The outcome of this study is quite promising in the aquaculture practice of this locally abundant edible freshwater crab which forms a cheap protein substitute for the malnourished tribes/poor people of Wayanad.
References
- Adiyodi, R. G.; Subramoniam, T. Arthropoda:
- Crustacea. In: Adiyodi, K. G.; Adiyodi, R. G.
- (Eds). Reproductive Biology of
- Invertebrates. Vol. I Oogenesis, oviposition
- and oosorption. New York: John Wiley and
- Sons, 1983. p. 443-495.
- Arvy, L.; Echalier, G.; Gabe, M. Modifications
- de la gonade de Carcinus maenas apres
- ablation bilaterale de l’organe Y. Comptes
- Rendus de l’Académie des Sciences, Serie
- D, v. 239, p. 1853-1855, 1954.
- Blanchet-Tournier, M. F. Quelques aspects
- des interactions hormonales entre la mue et
- la vitellogenèse chez le Crustacé Amphipode
- Orchestia gammarellus (Pallas).
- Reproduction Nutrition Development,
- v. 22, no. 2, p. 325-344, 1982.
- Brandau, H. Histochemical localization of
- enzyme activities in normal and
- gonadotrophin stimulated mouse ovaries. In:
- Butt, W. R.; Crooke, A. C.; Ryle, M. (Eds.).
- Gonadotrophins and Ovarian
- Development. Livingstone, Edinburgh,
- London: Eshkol, A., Lunenfeld, B., and Pet,
- p. 307-311.
- Brown, M.; Sieglaff, D.; Rees, H. Gonadal
- ecdysteroidogenesis in Arthropoda:
- Occurrence and regulation. Annual Review
- of Entomology, v. 54, p. 105-125, 2009.
- https://doi.org/10.1146/annurev.ento.53.10
- 093334
- Chaix, J. C.; De Reggi, M. Ecdysteroid levels
- during ovarian development and
- embryogenesis in the spider crab Acanthonyx
- lunulatus. General and Comparative
- Endocrinology, v. 47, no. 1, p. 7-14, 1982.
- https://doi.org/10.1016/0016-
- (82)90076-4
- Chan, S. M. Possible roles of 20-
- hydroxyecdysone in the control of ovary
- maturation in the white shrimp Penaeus
- vannamei (Crustacea: Decapoda).
- Comparative Biochemistry and
- Physiology - Part C: Toxicology &
- Pharmacology, v. 112, no. 1, p. 51-59, 1995.
- https://doi.org/10.1016/0742-8413(95)
- -9
- Chang, E. S. Hormonal control of molting in
- decapod Crustacea. American Zoologist,
- v. 25, no. 1, p. 179-185, 1985.
- https://doi.org/10.1093/icb/25.1.179
- Couch, E. F.; Hagino, N.; Lee. J. W. Changes in
- estradiol and progesterone immunoreactivity
- in tissues of the lobster, Homarus americanus,
- with developing and immature ovaries.
- Biochemistry and Physiology Part A:
- Molecular & Integrative Physiology, v. 87,
- no. 3, p. 765-770, 1987. http://doi.org/
- 1016/0300-9629(87)90397-5
- Crompton, D. W. Studies on the haemocytic
- reaction of Gammarus spp., and its
- relationship to Polymorphus minutus
- (Acanthocephala). Parasitology, v. 57, n. 2,
- p. 389-401, 1967. https://doi.org/10.1017/
- S0031182000072176
- Démeusy, N. Rôle de la gland de mue dans
- l'évolution ovarianne due crabe Carcinus
- Effect of ecdysteroids on oogenesis in the crab Travancoriana schirnerae 99
- Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 87-101.
- maenas Linn. Cahiers de Biologie Marine,
- v. 3, p. 37-56, 1962.
- Gong, J.; Ye, H.; Xie, Y.; Yang, Y.; Huang, H.; Li,
- S.; Zeng, C. Ecdysone receptor in the mud
- crab Scylla paramamosain: A possible role in
- promoting ovarian development. Journal of
- Endocrinology, v. 224, no. 3, p. 273-287,
- https://doi.org/10.1530/JOE-14-0526
- Gunamalai, V.; Kirubagaran, R.;
- Subramoniam, T. Sequestration of
- ecdysteroid hormone into the ovary of the
- mole crab, Emerita asiatica (Milne Edwards).
- Current Science, v. 85, no. 4, p. 493-496,
- Kale, R. S.; Shejule, K. B.; Kharat, P. S.;
- Sonawane, A. K. Induce ovarian maturation
- by 17α-hydroxyprogesterone hormone in
- freshwater crab, Barytelphusa cunicularis.
- Journal of Aquatic Biology, v. 23, no. 2,
- p. 109-112, 2008.
- Kale, R. S. Effect of β-estradiol hormone and
- eye stalk ablation on ovarian maturation in
- fresh water crab, Barytelphusa cunicularis.
- Bioscience Discovery, v. 8, no. 2, p. 140-145,
- Kulkarni, G. K.; Nagabhushanam, R.; Joshi, P.
- K. Effect of progesterone on ovarian
- maturation in a marine penaeid prawn
- Parapenaeopsis hardwickii (Miers, 1878).
- Indian Journal of Experimental Biology,
- v. 17, p. 986-987, 1979.
- Lachaise, F.; Hoffmann, J. A. Ecdysone et
- developement ovarien chez un Decapode,
- Carcinus maenas. Comptes Rendus de
- l’Académie des Sciences, Serie D, v. 285,
- p. 701-704, 1981.
- Lachaise, F.; Goudeau, M.; Hetru, C.; Kappler,
- C.; Hoffmann, J. A. Ecdysteroids and ovarian
- development in the shore crab, Carcinus
- maenas. Hoppe-Seyler’s Zeitschrift für
- Physiologische Chemie, v. 362, no. 1,
- p. 521-530, 1981. https://doi.org/10.1515/
- bchm2.1981.362.1.521
- Lanot, R.; Cledon P. Ecdysteroids and meiotic
- reinitiation in Palaemon serratus (Crustacea
- Decapoda Natantia) and in Locusta
- migratoria (Insecta Orthoptera): A
- comparative study. Invertebrate
- Reproduction & Development, v. 16,
- no. 1/3, p. 169-175, 1989. http://doi.org/
- 1080/07924259.1989.9672074
- Laufer, H.; Ahl, J. S.; Sagi, A. The role of
- juvenile hormones in crustacean
- reproduction. American Zoologist, v. 33,
- no. 3, p. 365-374, 1993. https://doi.org/
- 1093/icb/33.3.365
- Laufer, H.; Borst, D. W.; Foley, T. A.; Landau,
- M. Ecdysteroid titres in vitellogenic Libinia
- emarginata. Journal of Insect Physiology,
- v. 34, no. 7, p. 615-617, 1988.
- https://doi.org/10.1016/0022-1910(88)
- -6
- Medesani, D. M.; Ferre, L. E.; Canosa, I. S.;
- Silveyra, G. R.; Rodriguez, E. M. Induction of
- vitellogenesis by 17-hydroxyprogesterone
- and methyl farnesoate during postreproductive period, in the estuarine crab
- Neohelice granulata. Invertebrate
- Reproduction & Development, v. 59, no. 2,
- p. 1-7, 2015. http://doi.org/10.1080/
- 2015.1019014
- Merlin, J.; Mohanlal, D. L.; Balasubramanian,
- C. P.; Sherly, T.; Subramoniam, T.;
- Syamadayal, J.; Ravichandran, P.; Ponniah, A.
- G.; Gopal, C.; Vijayan, K. K. Induction of
- vitellogenesis and reproductive maturation
- in tiger shrimp, Penaeus monodon by 17ßestradiol and 17α-hydroxyprogesterone: In
- vivo and in vitro studies. Invertebrate
- Reproduction & Development, v. 59, no. 3,
- p. 166-175, 2015. http://doi.org/10.1080/
- 2015.1051192
- Mu, C.; Song, W.; Li, R.; Chen, Y.; Hao, G.;
- Wang, C. Identification of differentially
- expressed proteins relating to ovary
- development in Portunus trituberculatus.
- Aquaculture, v. 426, p. 148-153, 2014.
- https://doi.org/10.1002/jat.2965
- Muhd-Farouk, H.; Abol-Munafi, A.; Jasmani,
- S.; Ikhwanuddin, M. Effect of steroid
- hormones 17α-hydroxyprogesterone and
- α-hydroxypregnenolone on ovary external
- morphology of orange mud crab, Scylla
- olivacea. Asian Journal of Cell Biology, v. 9,
- p. 23-28, 2014. http://doi.org/10.3923/
- ajcb.2014.23.28
- Muhd-Farouk, H.; Jasmani, S.; Ikhwanuddin,
- M. Effect of vertebrate steroid hormones on
- the ovarian maturation stages of orange mud
- crab, Scylla olivacea (Herbst, 1796).
- Aquaculture, v. 451, p. 78-86, 2016.
- https://doi.org/10.1016/j.aquaculture.2015.
- 038
- Raghavan and Ayanath
- Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 87-101.
- Nagabhushanam, R.; Sambasivarao, S.;
- Sarojini, R.; Jayalakshmi, K. Annual
- reproductive cycle of female Metapenaeus
- affinis. Proceeding of the National
- Symposium on Physiology of Crustaceans,
- p. 39-41, 1987.
- Nagabhushanam, R.; Joshi, P. K.; Kulkarni, G.
- K. Induced spawning in prawn
- Parapenaeopsis stylifera (H. Milne Edwards)
- using a steroid hormone 17 hydroxyprogesterone. Indian Journal of Marine
- Sciences, v. 9, p. 227, 1980. http://doi.org/
- /39227
- Okumura, T.; Sakiyama, K. Hemolymph levels
- of vertebrate-type steroid hormones in
- female kuruma prawn Marsupenaeus
- japonicus (Crustacea: Decapoda: Penaeidae)
- during natural reproductive cycle and
- induced ovarian development by eyestalk
- ablation. Fisheries Science, v. 70, p. 372-
- , 2004.
- Okumura, T.; Aida, K. Fluctuations in
- hemolymph ecdysteroid levels during the
- reproductive and non-reproductive molt
- cycles in the giant freshwater prawn
- Macrobrachium rosenbergii. Fisheries
- Science, v. 66, no. 5, p. 876-883, 2000.
- https://doi.org/10.1046/j.1444-2906.2000.
- x
- Okumura, T.; Han, C. H.; Suzuki, Y.; Aida, K.;
- Hanyu, I. Changes in hemolymph vitellogenin
- and ecdysteroid levels during the
- reproductive and non-reproductive molt
- cycles in the freshwater prawn
- Macrobrachium rosenbergii. Zoological
- Science, v. 9, p. 37-45, 1992.
- Reddy, B. S.; Sridevi, V.; Neelima, H.; Reddy, P.
- R.; Reddy, P. S. Regulation of vitellogenesis by
- selected endocrine modulators in crab
- Oziothelphusa senex senex, with special
- reference to methyl farnesoate. Aquaculture
- Reports, v. 3, p. 24-30, 2016.
- https://doi.org/10.1016/j.aqrep.2015.11.006
- Reddy, P. R.; Kiranmayi, P.; Kumari, K.;
- Reddy, P. S. 17α-hydroxyprogesterone
- induced ovarian growth and vitellogenesis in
- the freshwater rice field crab Oziotelphusa
- senex senex. Aquaculture, v. 254, no. 1/4,
- p. 768-775, 2006. https://doi.org/10.1016/
- j.aquaculture.2005.11.023
- Rodríguez, E. M.; Medesani, D. A.; Greco, L. S.
- L.; Fingerman, M. Effects of some steroids
- and other compounds on ovarian growth of
- the red swamp crayfish, Procambarus clarkii,
- during early vitellogenesis. Journal of
- Experimental Zoology, v. 292, no. 1, p. 82-
- , 2002. https://doi.org/10.1002/jez.1144
- Sarojini, R.; Jayalakshmi, K.; Sambashivarao,
- S. Effect of external steroids on ovarian
- development in the freshwater prawn
- Macrobrachium lamerrii. Journal of
- Advanced Zoology, v. 7, no. 1, p. 50-53,
- Sarojini, R.; Mirajkar, M. S.; Nagabhushanam,
- R. Effect of steroids on the oogenesis and
- spermatogenesis of the freshwater prawn,
- Macrobrachium kistnensis. Comparative
- Physiology and Ecology, v. 10, no. 1, p. 7-11,
- Sarojini, R.; Sambasivarao, S.; Jayalakshmi, K.
- Effect of steroids (Estradiol and estrone) on
- the ovaries of the marine crab, Scylla serrata.
- Comparative Physiology and Ecology,
- v. 15, no. 1, p. 21-26, 1990.
- Steel, C. G. H.; Vafopoulou, X. Ecdysteroid
- titres in haemolymph and other tissues
- during moulting and reproduction in the
- terrestrial isopod, Oniscus asellus (L.).
- Invertebrate Reproduction &
- Development, v. 34, no. 2/3, p. 187-194,
- https://doi.org/10.1080/07924259.
- 9652652
- Subramoniam, T. Crustacean ecdysteroids in
- reproduction and embryogenesis.
- Comparative Biochemistry and
- Physiology - Part C: Toxicology &
- Pharmacology, v. 125, no. 2, p. 135-156,
- https://doi.org/10.1016/S0742-
- (99)00098-5
- Sujathamma, A.; Dayakar, Y. Effect of
- estradiol and 17α-hydroxyprogesterone on
- ovarian development of freshwater paddy
- field crab, Oziotelphusa senex senex
- (Fabricius). European Journal of
- Experimental Biology, v. 5, n. 9, p. 27-30,
- Swetha, C. H.; Girish, B. P.; Reddy, P. S.
- Elucidation of the role of estradiol and
- progesterone in regulating reproduction in
- the edible crab, Oziothelphusa senex senex.
- RSC Advances, v. 6, no. 30, p. 24959-24967,
- https://doi.org/10.1039/C5RA23637A
- Tsukimura, B.; Kamemoto, F. L. Organ culture
- assay of the effects of putative reproductive
- hormones on immature penaeid ovaries. The
- Effect of ecdysteroids on oogenesis in the crab Travancoriana schirnerae 101
- Braz. J. Biol. Sci., 2019, Vol. 6, No. 12, p. 87-101.
- World Aquaculture Society Abstract,
- v. 288, 1988.
- Van Herp, F.; Payen, G. G. Crustacean
- neuroendocrinology: Perspectives for the
- control of reproduction in aquacultural
- systems. Bulletin of the Institute of
- Zoology, Academia Sinica, v. 16, p. 513-539,
- Wongsawang, P.; Phongdara, A.; Chanumpai,
- A.; Chotigeat, W. Detection of CHH/GIH
- activity in fractionated extracts from the
- eyestalk of Banana prawn. Songklanakarin
- Journal of Science and Technology, v. 27,
- p. 789-798, 2005.
- Yano, I. Effect of 17-hydroxy-progesterone on
- vitellogenin secretion in kuruma prawn,
- Penaeus japonicus. Aquaculture, v. 61, p. 49-
- , 1987.
- Young, N. J.; Webster, S. G.; Rees, H. H.
- Ecdysteroid profiles and vitellogenesis in
- Penaeus monodon (Crustacea: Decapoda).
- Invertebrate Reproduction &
- Development, v. 24, no 2, p. 107-117. 1993.
- https://doi.org/10.1080/07924259.1993.96
- Yudin, A. I.; Diener, R. A.; Clark, Jr. W.; Chang,
- E. S. Mandibular gland of the blue crab,
- Callinectes sapidus. The Biological Bulletin,
- v. 159, p. 760-772, 1980.
- Zapata, V.; Lopez Greco, L. S.; Medesani, D.;
- Rodriguez, E. M. Ovarian growth in the crab,
- Chasmagnathus granulata induced by
- hormones and neuroregulators throughout
- the year: In vivo and in vitro studies.
- Aquaculture, v. 224, no. 1/4, p. 339. 2003.
- http://doi.org/10.1016/S0044-8486(03)
- -6
