First ever report on the antimicrobial activities of some selected mangrove halophytes of Sundarban against milk spoilage microflora

Resumen

The study was governed by the objective of investigating
the antimicrobial effects of mangrove leaf extracts. The fresh and
dried leaf extracts from Avicennia marina (Forssk.) Vierh.,
Avicennia officinalis L., Aegiceras corniculatum (L.) Blanco,
Aegialitis rotundifolia Roxb., Acanthus ilicifolius L, Lumnitzera
racemosa Willd., Excoecaria agallocha L. and Sonneratia apetala
Buch-Ham. were found to have inhibitory effects on the milk
spoilage microflora obtained from curd sample. Methanol and
Acetone were used as extraction solvents and in comparison,
extracts by acetone were observed to suppress the microbial growth
to lesser extents. Leaf extracts of A. marina, A. officinalis L.
racemosa and A. rotundifolia were able to exert relatively greater
inhibitory effect on the milk spoilage microbial broth than others,
although all the species expressed some degree of suppression.
Interestingly A. rotundifolia, S. apetala, L. racemosa and
E. agallocha demonstrated greater efficiency in formation of larger
growth inhibition zones on the petri plates. The extracts obtained
from dry leaves produced greater adverse effects of the microbes
than the raw leaf extracts. The data generated were analyzed with the
help of ‘ANOVA: Single factor’ and ‘F-test: Two sample for
variance’ and data sets were of statistical significance at 0.05 level
of significance as null hypothesis was rejected.

Citas

1. Abeysinghe, P. D.; Vithanawasam, M.;
2. Pathirana, R. N.; Abeysinghe, S. Preliminary in
3. vitro screening of some mangrove plant extracts
4. for antibacterial compounds against clinical
5. bacterial isolates from different sources. 1st
6. Science Symposium Proceeding, v. 1,
7. p. 22-25, 2000.
8. Agoramoorthy, G.; Chandrasekaran, M.;
9. Venkatesalu, V.; Hsu, M. J. Antibacterial and
10. antifungal activities of fatty acid methyl esters
11. of the blind-your eye mangrove from India.
12. Brazilian Journal of Microbiology, v. 38,
13. p. 739-742, 2007. http://doi.org/10.1590/S1517
14. Aliero, A.; Aliero, B. L.; Buhari, U. Preliminary
15. phytochemical and antibacterial screening of
16. Scadoxus multiflorus. International Journal of
17. Pure and Applied Science, v. 2, p. 13-17,
18. Alizadeh-Behbahani, B.; Tabatabaei-Yazdi, F.;
19. Shahidi, F.; Mohebbi, M. Antimicrobial activity
20. of Avicennia marina extracts ethanol, methanol
21. & glycerin against Penicillium digitatum (citrus
22. green mold). Scientific Journal of
23. Microbiology, v. 1, No. 7, p. 147-151, 2012.
24. Available
25. from:
26. <http://sjournals.com/
27. index.php/SJMi/article/view/406/pdf_1>.
28. Accessed on: May 25, 2017.
29. Bandaranayake, W. M. Bioactivities, bioactive
30. compounds and chemical constituents of
31. mangrove plants. Wetlands Ecology and
32. Management, v. 10, no. 6, p. 421-452, 2002.
33. https://doi.org/10.1023/A:1021397624349
34. Bandaranayake, W. M. Economic, traditional
35. and medicinal uses of mangroves. Townsville:
36. Australian Institute of Marine Science, 1999.
37. (AIMS Report, No. 28).
38. Bandaranayake, W. M. Survey of mangrove
39. plants
40. from
41. Northern
42. Australia
43. for
44. phytochemical constituents and UV-absorbing
45. compounds.
46. Current
47. Topics
48. Phytochemistry, v. 14, p. 60-72, 1995.
49. in
50. Bhimba, B. V.; Meenupriya, J.; Joel, E. L.;
51. Naveena, D. E.; Kumar, S.; Thangaraj, M.
52. Antibacterial activity and characterization of
53. secondary metabolites isolated from mangrove
54. plant Avicennia officinalis. Asian Pacific
55. Journal of Tropical Medicine, v. 3, p. 544
56. ,
57. (10)60131-9
58. https://doi.org/10.1016/S1995
59. Biswas, H.; Dey, M.; Ganguly, D.; De, T. K.;
60. Ghosh, S.; Jana, T. K. Comparative analysis of
61. phytoplankton composition and abundance over
62. a two-decade period at the land-ocean boundary
63. of a tropical mangrove ecosystem. Estuaries
64. and Coasts, v. 33, No. 2, p. 384-394, 2010.
65. https://doi.org/10.1007/s12237-009-9193-5
66. Chandrasekaran,
67. M.;
68. Kannathasan,
69. K.;
70. Venkatesalu, V.; Prabhakar, K. Antibacterial
71. activity of some salt marsh halophytes and
72. mangrove plants against methicillin resistant
73. Staphylococcus aureus. World Journal of
74. Microbiology and Biotechnology, v. 2, p. 155
75. , 2009. https://doi.org/10.1007/s11274-008
76. -1
77. Gazim, Z. C.; Rezende, C. M.; Fraga, S. R.;
78. Svidzinski, T. I.; Cortez, D. A. Antibacterial
79. activity of the essential oil from Calendula
80. officinalis L. (Asteraceae) growing in Brazil.
81. Brazilian Journal of Microbiology, v. 39,
82. p. 61-63,
83. http://dx.doi.org/10.1590/
84. S1517-83822008000100015
85. Gulshan,
86. N.;
87. Radhakrishnan,
88. M.;
89. Balagurunathan, R. Antimicrobial compounds
90. from certain Indian medicinal plants against
91. multi drug resistant bacteria. Indian Jounal of
92. Applied Microbiology, v. 1, no. 1, p. 23-28,
93. Jacquelyn, G. B. Microbiology principles and
94. exploration. 5. ed. New York: John Wiley and
95. Sons, 2002.
96. Braz. J. Biol. Sci., 2017, v. 4, No. 8, p. 273-292.
97. Antimicrobial activities of some mangrove halophytes against milk spoilage microflora
98. Kokpol, U.; Chittawong, V.; Mills, H. D.
99. Chemical constituents of the roots of Acanthus
100. ilicifolius. Journal of Natural Products, v. 49,
101. no. 2, p. 355-356, 1984. https://doi.org/10.1021/
102. np50044a033
103. Laith, A. A.; Najiah, M. Antimicrobial activities
104. of blinding tree, Excoecaria agallocha against
105. selected bacterial pathogens. Journal of
106. Microbiology and Antimicrobials, v. 6, no. 2,
107. p. 29-36,
108. JMA2013.0291
109. https://doi.org/10.5897/
110. Littleton, J.; Rogers, T.; Falcone, D. Novel
111. approaches to plant drug discovery based on
112. high throughput pharmacological screening and
113. genetic manipulation. Life Sciences, v. 78,
114. no. 5, p. 467-475, 2005. https://doi.org/10.1016/
115. j.lfs.2005.09.013
116. Mojab, F.; Poursaeed, M.; Mehrgan, H.;
117. Pakdaman, S. Antibacterial activity of Thymus
118. daenensis methanol extract. Pakistan Journal
119. of Pharmacology Science, v. 21, p. 210-213,
120. Morales, G.; Paredes, A.; Sierra, P.; Loyola, L.
121. A. Antimicrobial activity of three baccharis
122. species used in the traditional medicine of
123. Northern Chile. Molecules, v. 13, p. 790-794,
124. http://dx.doi.org/10.3390/molecules130
125. Mukherjee, A.; Das, S.; Chakraborty, S.; De, T.
126. K. An ex situ and in vitro approach to delineate
127. pennate diatom species with bioindicator
128. potentials in a well mixed tropical estuarine
129. ecosystem. Brazilian Journal of Biological
130. Sciences, v. 3, no. 6, p. 299-317, 2016.
131. https://doi.org/10.21472/bjbs.030607
132. Mukhopadhyay, S. K.; Biswas, H.; De, T. K.;
133. Jana, T. K. Fluxes of nutrients from the tropical
134. River Hoogly at the land-ocean boundary of
135. Sundarban, NE coast of Bay of Bengal. Journal
136. of Marine Systems, v. 62, p. 9-21, 2006.
137. https://doi.org/10.1016/j.jmarsys.2006.03.004
138. Nascimento, G. G. F.; Locatelli, J.; Freitas, P.
139. C.; Silva, G. L. Antibacterial activity of plant
140. extracts and phytochemicals on antibiotic
141. resistant
142. bacteria.
143. Microbiology,
144. Brazilian Journal of
145. v. 31,
146. p. 247-256,
147. https://doi.org/10.1590/S1517-838220000004
148. Nebedum, J.; Ajeigbe, K.; Nwobodo, E.; Uba,
149. C.; Adesanya, O.; Fadare, O.; Ofusori, D.
150. Comparative study of the ethanolic extracts of
151. four Nigerian plants against some pathogenic
152. microorganisms. Res. J. Med. Plant, v. 3, p.
153. -28,
154. rjmp.2009.23.28
155. http://dx.doi.org/10.3923/
156. Opra, E. U.; Wokocha, R. C. Efficacy of some
157. plant extracts on the in vitro and in vivo control
158. of Xanthomonas campestris pv. vesicatoria.
159. Agricultural Journal, v. 3, p. 163-170, 2008.
160. Panda, S. K.; Thatoi, H. N.; Dutta, S. K.
161. Antibacterial
162. activity
163. and
164. phytochemical
165. screening of leaf and bark extracts of Vitex
166. negundo I. from Similipal Biosphere Reserve,
167. Orissa. Journal of Medicinal Plants Research,
168. v. 3, p. 294-300, 2009. Available from:
169. <http://www.academicjournals.org/article/articl
170. e1380377015_Panda et al.pdf>. Accessed on:
171. May 23, 2017.
172. Powar, P.; Gaikwad, D. GC-MS Analysis of
173. bioactive compounds of Aegiceras corniculatum
174. bark. Indian Journal of Plant Sciences, v. 5,
175. no. 3, p. 13-17, 2016. Available from:
176. <http://www.cibtech.org/J-Plant
177. Sciences/PUBLICATIONS/2016-VOL/JPS
178. VOL-5-NO-3/03-JPS-003-PRATISHTHA
179. ANALYSIS.pdf>. Accessed on: May 23, 2017.
180. Rajkrishnan, A.; Ponnusamy, K. Antifungal
181. activity of Clerodendrum inerme (L.) and
182. Clerodendrum phlomidis (L.). Turkian Journal
183. of Biology, v. 30, p. 139-142, 2006. Available
184. from:
185. <http://journals.tubitak.gov.tr/biology/
186. issues/biy-06-30-3/biy-30-3-4-0510-4.pdf>.
187. Accessed on: May 23, 2017.
188. Saad, S.; Taher, M.; Susanti, D.; Qaralleh, H.;
189. Izyani, A. F. Awang, Bt. In vitro antimicrobial
190. activity of mangrove plant Sonneratia alba.
191. Asian
192. Pacific
193. Journal
194. of
195. Tropical
196. Biomedicine, v. 2, no. 6, p. 427-429, 2012.
197. https://dx.doi.org/10.1016/S2221-1691(12)
198. -0
199. Saad, S.; Taher, M.; Susanti, D.; Qaralleh, H.;
200. Izyani, A. F.; Awang, Bt. Antimicrobial activity
201. of mangrove plant (Lumnitzera littorea). Asian
202. Pacific Journal of Tropical Biomedicine, v. 4,
203. no. 7, p. 523-525, 2011. https://doi.org/10.1016/
204. S1995-7645(11)60138-7
205. Saravanan,
206. D.;
207. Radhakrishnan,
208. M.
209. Antimicrobial activity of mangrove leaves
210. against drug resistant pathogens. International
211. Journal of Pharm Tech Research, v. 9, no. 1,
212. p. 141-146,
213. Available
214. from:
215. <http://sphinxsai.com/2016/ph_vol9_no1/1/(141-146)V9N1PT.pdf>. Accessed on: May 23,
216. Sayyed,
217. H.;
218. Yogita,
219. P.;
220. Javesh,
221. P.;
222. Lakshmikant, B.; Sunil, P.; Goldee, S. P.
223. Antibacterial and antifungal potential of
224. Clerodendrum inerme crude extracts against
225. some human pathogenic microorganism.
226. Pharmacology Online, v. 2, p. 75-79, 2008.
227. Braz. J. Biol. Sci., 2017, v. 4, No. 8, p. 273-292.
228. Mukherjee et al.
229. Braz. J. Biol. Sci., 2017, v. 4, No. 8, p. 273-292.
230. Sharief, Md. N.; Rao, U. V. Antibacterial
231. activity of stem and root extracts of Avicennia
232. Officinalis L. International Journal of
233. Pharmaceutical Applications, v. 2, no. 4,
234. p. 231-236, 2011. Available from:
235. <https://bipublication.com/files/IJPAv2i42011
236. pdf>. Accessed on: May 23, 2017.
237. Teja, V. P.; Ravishankar, K. Preliminary
238. phytochemical investigation and in vitro
239. antimicrobial activity of ethanolic extracts of
240. Sonneratia apetala plant. International
241. Research Journal of Pharmacy, v. 4, no. 6,
242. p. 84-87, 2013. https://doi.org/10.7897/2230
243. 04619
244. Vadlapudi, V.; Naidu, K. C. Bioactivity of
245. marine mangrove plant Avicennia alba on
246. selected plant and oral pathogens. International
247. Journal of ChemTech Research, v. 1, no. 4,
248. p. 1213-1216, 2009. Available from:
249. <http://sphinxsai.com/CTVOL4/ct_pdf_vol_4/C
250. T =69 (1313-1316).pdf>. Accessed on: May 23,